Berries are known to contain polyphenols, including phenolic acids, and known Sararanga sinuosa fruits are classified in berries. Identification phenolic acids of S. sinuosa fruit extract was done by LC-ESI-Orbitrap-MS/MS. The prediction of interaction and potency of antiglycation of phenolic acid with the protein surface of human serum albumin (HSA) was done by molecular docking. The phenolic acids in S. sinuosa fruit extracts were syringic acid, isovanillic acid (hydroxybenzoic acid group), chlorogenic acid, 2-hydroxycinnamic acid, sinapinic acid, caffeic acid, and ferulic acid (hydroxycinnamic acid group). Chlorogenic acid, caffeic acid, syringic acid, 2-hydroxycinnamic acid, and synapinic acid binds to HSA at FA7 site (subdomain IIA). Whereas, isovanillic acid and ferulic acid bind to FA6 (between subdomains IIA and IIB) and FA1 sites (subdomain IA), respectively. Phenolic acids that bind to the amino acid residues of Lys195 and/or Lys199, namely chlorogenic acid, caffeic acid, syringic acid, 2-hydroxycinnamic acid, and sinapinic acid, predicted to occupy the sites for the glycation reaction in HSA. In conclusion, S. sinuosa fruit extract contains seven phenolic acids that can bind to HSA. Five of them, namely chlorogenic acid, caffeic acid, syringic acid, 2-hydroxycinnamic acid, and sinapinic acid, predictable have the potential to play a role in antiglycation.
Key words: Antiglycation, Sararanga sinuosa, Molecular docking, Pandan anggur, Phenolic acids
|
