Objective of this study was to determine cytotoxicity and antimicrobial effects of SA-PEG, SA-PEG-OCT, CMC-PEG,
CMC-PEG-OCT, SA-CMC-PEG and SA-CMC-PEG-OCT hydrogels for wound healing application. In this study, the cytotoxicity
effect were observed using human foreskin fibroblast (HS-27) cell that is treated with concentration of hydrogels’ extract of 25%,
50% and 100% for 24 hours. The cytotoxic activity was determined via CellTiter®-Blue cell viability assay. Meanwhile, disk
diffusion method was used using Staphylococcus aureus to observe the Zone of Inhibition (ZOI) of hydrogels for antimicrobial
study. Cell viability assay revealed that the results of mean cell viability for 100% hydrogel concentration according to decreasing
order are as follow: SA-PEG-OCT > SA-CMC-PEG-OCT > SA-PEG > SA-CMC-PEG > CMC-PEG > CMC-PEG-OCT. All
hydrogels loaded with OCT showed ZOIs that manifested antimicrobial effects against S. aureus. However, there is no ZOIs
observed for hydrogels without OCT. The observed SA-PEG-OCT hydrogel, CMC-PEG-OCT hydrogel and SA-CMC-PEG-OCT
hydrogel that contain approximately 0.5% OCT each demonstrated antimicrobial activity against S. aureus without undesirable
cytotoxicity on fibroblast cell. Thus, SA/CMC based hydrogel loaded with OCT may be suitable for the use in wound healing
management.
Key words: : hydrogel, cytotoxicity, antimicrobial, sodium alginate, carboxymethyl cellulose, octenidine dihydrochloride, antiseptic
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