The aim of this study is to evaluate the in vitro anti-inflammatory effect of Mikania micrantha (L.) Kunth. leaf extract on RAW 264.7 murine leukemia cell line. The qualitative phytochemical analysis of the different extracts of the leaves of M. micrantha revealed the presence of carbohydrates, flavonoids, quinones, terpenoids, phenols, and coumarins, whereas the quantification revealed that the methanol extract contained the highest phenol content (259.88 mg catechol equivalent/g dried sample) as well as flavonoid content (156.55 mg quercetin equivalent/g of dried sample). The different extracts were tested for antioxidant activity using a DPPH scavenging assay. The antioxidant capacity of ethyl acetate extract at 100 µg concentration showed the highest DPPH scavenging ability with an IC50 value of 40.34 µg/ml in comparison with the standard (39.92 µg/ml). Allium cepa assay and MTT assay were performed to assess the cytotoxicity effects. The fresh leaf extract increased the incidence of anomalous mitosis. Cytotoxicity study showed that ethyl acetate extracts exhibit the highest cytotoxicity with an IC50 value of 47.68 µg/ml. Reverse-transcription polymerase chain reaction analysis exhibited the suppression of the RAGE gene. This is the first report on the effect of the anti-inflammatory activity of M. micrantha leaf extract on RAW 264.7 murine leukemia cell line. This study concluded that M. micrantha possesses antioxidant property and limiting RAGE gene expression suggests anti-inflammatory properties.
scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.
The articles in Bibliomed are open access articles licensed under Creative Commons Attribution 4.0 International License (CC BY), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
We use cookies and other tracking technologies to work properly, to analyze our website traffic, and to understand where our visitors are coming from. More InfoGot It!
