In this study, phytochemical screening, docking study, and cytotoxicity against MCF7 and 3T3 cell lines of Indonesian sea cucumber Stichopus sp. fractions were investigated. The total phenol content and phytochemicals screening of these samples were ascertained by a fraction conventional method. The thiazolyl blue tetrazolium bromide assay was applied to determine the effect of Stichopus sp. fractions on cell viability. Phytochemical screening exhibited positive results for alkaloids, steroids, flavonoids, saponins, and triterpenoids. The highest amount of phenol content was discovered in the hexane fraction, and the lowest was in the sediment. A molecular docking study was conducted to examine the interaction between the Stichopus sp. chemical compounds and the MMP-9 cancer protein. The results of the in vitro assessment of cytotoxicity against the MCF7 and 3T3 cell lines of Indonesian sea cucumber Stichopus sp. clearly revealed that all of the fractions are toxic for cancerous MCF7 cell lines and most fractions are not toxic for non-cancerous 3T3 cell lines (normal cell lines). The in vitro assessment of cytotoxicity against the MCF7 and 3T3 cell lines showed that Stichopus sp. fractions have the ability to be a potential candidate for anticancer drugs.
Key words: Stichopus sp, sea cucumber, phytochemical, MCF7, 3T3, cell lines, molecular docking
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