Abstract

The Bajakah tampala plant (Spatholobus littoralis Hassk) is one of the plants that grows in Indonesia, precisely on the
island of Borneo, which has antibacterial activity but its antibiofilm activity against Pseudomonas aeruginosa ATCC 27853 has
never been reported. The search for new antibiofilms against P. aeruginosa biofilms is important in preventing biofilm-associated
infections. This study aimed to determine the effectiveness of Bajakah tampala (Spatholobus littoralis Hassk) ethanol extract in
inhibiting the formation of P. aeruginosa biofilm. The planktonic and biofilm inhibition tests were carried out using the microtiter
broth method. Antibiofilm activity of Bajakah tampala (Spatholobus littoralis Hassk) ethanol extract against P. aeruginosa was
analyzed by calculating the minimum biofilm inhibitory concentration (MBIC50) and biofilm eradication activity were analyzed by
calculating the minimum biofilm eradiaction concentration (MBEC50). The ethanol extract of Bajakah tampala (Spatholobus
littoralis Hassk) 1% gave P. aeruginosa antibacterial activity of 83.20% ± 0.01 and mid-phase antibiofilm activity of 79.30% ±
0.01, maturation phase 76.30 % ± 0.01 and eradication activity of 70.77 % ± 0.01. Scanning electron microscopy results also
showed that the ethanol extract of Bajakah tampala (Spatholobus littoralis Hassk) could damage the matrix of extracellular
polymeric substances biofilm P. aeruginosa. Therefore, the ethanol extract of Bajakah tampala (Spatholobus littoralis Hassk) has
the potential to be developed as a candidate for new antibiofilm drugs against P. aeruginosa biofilms.

Key words: Bajakah Tampala, Biofilms, Pseudomonas aeruginosa, Spatholobus littoralis Hassk