The treatment of polluted sites has become a priority for the community due to the increase in quality of
life standards, the development of technology and awareness of environmental issues. Biological methods were
chosen to treat these pollutants. The samples were collected from the central oil company, which is a polluted site
with petroleum hydrocarbons,The analysis focused based on Polymerase Chain Reaction PCR technology before
and after treatment and with ten primers to detect oil mutations due to contamination. The plants gave different
genetic variations confirmed by the percentage of the genetic distance resulting from the analysis of the results of
the RAPD indicators, which reached the highest 40% between the polluted and treated Alhagi graecorum plants.
The current study proved the efficiency of activated carbon in the treatment of petroleum hydrocarbons .
Key words: RAPD PCR in plant , Anti-Pollution, Crude oil
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