Invasive fungal infections (IFIs) have emerged in the last three decades as an important cause of human disease. These infections treated particularly with triazole such as fluconazole, itraconazole and voriconazole but some serious adverse reactions have been reported in many studies. In vitro activities of 12 essential oils were evaluated against 6 clinically important fungi. Thyme oil was the most effective essential oil against all the tested fungi and Aspergillus flavus was the most susceptible with inhibition zone diameter and MIC value 39 mm and 0.25 µg/ml, respectively. Active ingredients of thyme oil were analyzed using GC/Mass chromatography and extracted by Thin Layer Chromatography (TLC). Thymol was the most active component against Aspergillus flavus with inhibition zone diameter and MIC value 44 mm and 0.125 µg/ml, respectively. The inhibitory activity of thymol against Aspergillus flavus was confirmed using Transmission electron microscope.
Key words: Thymol, Invasive fungal infections, Aspergillus flavus, GC/Mass, Essential oils, Antifungals, Thyme oil.
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