Broken Riceberry rice powder was saccharified by ultrasound-assisted enzymatic hydrolysis for the production of sugar syrup containing phenolic compounds. The results showed that total soluble solid (TSS) content at 17.0 ± 0.5°Brix was obtained when sonicating broken Riceberry rice powder (250 g/l) with a low-temperature amylase at 60% amplitude for 20 minutes and subsequent hydrolysis at 50o C for 12 hours. Upscaled hydrolysis in a 5.0 l reactor gave the highest TSS content and total phenolic content (TPC) at 16.33 ± 0.29°Brix and 5.63 ± 0.12 mg GAE/g sample, respectively. The obtained Riceberry syrup was used for bacterial cellulose (BC) fermentation using Komagataeibacter xylinus AGR 60, which was incubated at room temperature for 4 days and yielded 4.80 ± 0.30 mm of thickness with 2.51 ± 0.24 g of dry weight equivalent to 3.14 ± 0.30 g/l/day. The physical structure of the obtained BC showed a crowded cellulose network, which showed the potential for water holding capacity of a facial mask sheet product. The five facial mask formulations were tested and showed significant results for the storage ability. This study provided an innovative product from the low-cost agricultural crop through the biotechnological process and showed the potential for further application at the commercial level.
Key words: Broken Riceberry rice, Facial mask product, Ultrasound, Bacterial cellulose, Enzymatic hydrolysis
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