Home|Journals|Articles by Year Follow on Twitter

Directory for Medical Articles
 

Open Access

Original Article



HPLC-FTIR spectroscopy combined with multivariate calibration for analysis of Andrographolide in Andrographis paniculata extract

Abdul Rohman, Hanifah Luthfianasari, Irnawati, Sugeng Riyanto, Mohamad Rafi, Bambang Prajogo, Muhammad Bachri Amran.

Abstract
Andrographis paniculata, known as Sambiloto in Indonesia, has been reported to have pharmacological activities with the main active constituent being andrographolide (ANDR). The present study highlighted the research for determining the levels of ANDR by correlating the absorbances of Fourier transform infrared (FTIR) spectra with ANDR contents as quantitatively analyzed by reference method of High-performance liquid chromatography (HPLC) using multivariate calibrations. Andrographis paniculata herbs from several regions were powdered. The powdered samples were measured in terms of FTIR spectra. Besides, a weighted sample was subjected to the extraction procedure and measured using HPLC. Data obtained are subjected to multivariate calibrations. The result indicated that the method was useful to evaluate ANDR content in A. paniculata herb. Partial least square regression (PLSR) using FTIR spectra in the form of a second derivative at the wavenumber regions of 3,700665 cm−1 was finally preferred for the quantitative analysis of ANDR with Coefficient of determination (R2 ) values of 0.9997 in the calibration model and 0.9765 in the validation models. The values of Root mean square error of calibration (RMSEC) and Root mean square error of prediction (RMSEP) obtained were 0.005 and 0.055, respectively. Due to its capability of providing a high value of R2 and low values of RMSEC and RMSEP, the application of PLSR using the variable of FTIR spectra at selected conditions could be an effective alternative method for quantitative analysis of ANDR.

Key words: Andrographis paniculata, FTIR spectra, HPLC, Partial Least Square Regression, Principal Component Regression



Similar Articles

Characterization of Covid-19 infected pregnant women sera using laboratory indexes, vibrational spectroscopy, and machine learning classifications.
Guleken Z, Jakubczyk P, Wiesław P, Krzysztof P, Bulut H, Öten E, Depciuch J, Tarhan N
Talanta. 2022; 237(): 122916

Applicability of two-stage anoxic/oxic shortcut nitrogen removal via partial nitrification and partial denitrification for municipal wastewater by adding sludge fermentation products continuously.
Chen Y, Zhang Y, Zhang L, Zhang S, Peng Y
Chemosphere. 2022; 287(Pt 2): 132053

Socioeconomic Status and Individual Personal Responsibility Beliefs Towards Food Access.
Coleman RA, Fulford MD
Food ethics. 2022; 7(1): 1

Corrigendum to "Data mining Raman microspectroscopic responses of cells to drugs in vitro using multivariate curve resolution-alternating least squares" [Talanta 208 (2020) 120386].
Perez-Guaita D, Quintas G, Farhane Z, Tauler R, Byrne HJ
Talanta. 2022; 236(): 122682

Choice of ABO Group for Blood Component Transfusion in ABO-Incompatible Solid Organ Transplantation: A Questionnaire Survey in Korea and Guideline Proposal.
Chung Y, Ko DH, Lim J, Kim KH, Kim H
Annals of laboratory medicine. 2022; 42(1): 105-109


Full-text options


Latest Statistics about COVID-19
• pubstat.org


Add your Article(s) to Indexes
• citeindex.org






Covid-19 Trends and Statistics
ScopeMed.com
CiteIndex.org
CancerLine
FoodsLine
PhytoMedline
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.



ScopeMed Web Sites