Abstract

The hydroxylated chalcone derivative (HCD) has been synthesized efficiently utilizing of Claisen-Schmidt
condensation of para-hydroxylated derivatives of both acetophenone and benzaldehyde using a catalyst SOCl2/ETOH. The
confirmation and characterization of the chemical structure of the synthesized chalcone derivative was done using melting
points, and Rf value as well as FT-IR. The in vitro anti‐inflammatory activity for the synthesized (HCD) was evaluated at
different concentrations via Red Blood Cells membrane stabilization test and calculate IC50 value by linear regression analysis.
Additionally, Ibuprofen was used as a standard drug in this study. The screening data indicated that tested HCD showed potent
anti-inflammatory activity in comparison with Ibuprofen with the IC50 value of 1146.78±0.55μg/ml. The marked antiinflammatory potential of (HCD) has been found as it effectively inhibits heat induced hemolysis in comparison to ibuprofen.
The gained results encouraged to use the synthetic (HCD) as an effective anti-inflammatory agent instead of NSAIDs to avoid
classical side effects of NSAIDs.

Key words: NSAIDs, Ibuprofen, hydroxylated chalcone derivative, anti-inflammatory activity