Aim: We intended to assess the hepatoprotective effects of phenethyl isothiocyanate (PEITC) against oxidative liver injury induced by a high-fat diet (HFD) and streptozotocin (STZ) diabetes through the Nuclear Factor E2-Related Factor 2 (Nrf2) and Sirtuin 1 (SIRT1) pathways in rats.
Materials and Methods: Thirty male Wistar Albino rats were separated into three groups: the control group, the second group (HFD+STZ) fed HFD and injected with STZ (35mg/kg b.w.), and the third group (HFD+ STZ+PEITC) fed an HFD, injected with STZ (35mg/kg b.w.), and given PEITC (40mg/kg b.w. by oral gavage). Feeding with HFD and PEITC was given for two weeks and continued one more week following STZ. Serum ALT and lipids levels, antioxidant enzyme activities, MDA, GST, SIRT1, NF-ƙβ, and Nrf2 levels were measured. Liver histological changes were detected.
Results: In comparison with the control group, in the HFD+STZ group, serum HDL levels, activities of hepatic antioxidant enzymes, Nrf2 activity, levels of GST, SIRT1, and NF-ƙβ reduced and besides, serum ALT, TG, TC, LDL / VLDL, and hepatic MDA levels increased (p
Key words: Phenethyl isothiocyanate; Nuclear Factor E2-Related Factor 2; Sirtuin 1; liver injury
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