The distribution of plants on the planet is associated with high temperatures as a limiting factor. Indian almond seedlings were planted in pots subjected to ascorbic acid (AsA) and calcium chloride alone or combined to reduce the negative impact of high field temperature. All treatments improved growth parameters compared with the control. The combined treatments increased chlorophyll content. In contrast, AsA 100 combined with calcium chloride 500 increased carotenoid pigment. However, the treatments modulated proline, total soluble carbohydrate, phenolic, and AsA contents. Heat stress increased abscisic acid (ABA) content and electrolyte leakage percentage, whereas calcium decreased. AsA 50 mg/L decreased ABA and electrolyte leakage, whereas calcium increased. The treatments modulated protein profile in the number of proteins separated into bands on the gel. The thickness and density of these bands indicate differences in the molecular weights of proteins by treatments. The seedlings responded to heat stress during the 2nd week in July 2021, and the control treatment was more responsive to the temperature rise than the other treatments. The adaptation of the Indian almond plants to high temperatures during the summer in semi-tropical regions is possible using AsA and calcium chloride to reduce heat stress damage on the plant.
Key words: Adaption, Ascorbic acid, Calcium chloride, Heat stress, Electrolyte leakage, Protein pattern.
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