A present study aimed at preparation and evaluation of Lapatinib loaded polymeric micelles for the better treatment of breast cancer. LP-PMs were prepared as per our previous studies by using Soluplus® as polymer. Therefore, we employed a lyophilization technique using mannitol as cryoprotectant and further conducted in-vitro and in-vivo anti-cancer efficacy studies, in addition to our previously reported work. We found that the lyophilized LP-PMs were sufficiently stable and retained encapsulated drug. Further, their smooth surface was visualized on atomic force microscopy. The X-ray powder diffractogram of LP-PMs showed successful encapsulation of LP; however presence of few drug molecules on surface was evidenced by energy dispersive X-ray analysis. Further, LP-PMs showed sustained release of drug with selective drug release in an acidic environment, resembling that of tumor. The LP-PMs exhibited higher cytotoxicity against SKBr3 breast cancer cells and also induced effective inhibition of growth of tumor in-vivo as compared to that of lapatinib solution and marketed formulation. The results of the present study indicated the greater potential of LP-PMs for the efficient treatment for breast cancer.
Key words: Anti-cancer Agent, Nanocarrier, Passive Targeting, Polymeric Micelles, SKBr3.
|
