Study on the evaluation of Acanthaster planci, Echinaster luzonicus and Echinotrix calamaris from Peninsular Malaysia for cytotoxicity against human hepatocellular liver carcinoma cells (HepG2), antioxidant and antibacterial activities were evaluated on their methanol extracts. The cytotoxicity and antioxidant, and antibacterial activities were conducted by MTS assay, DPPH free radical scavenging assay, disc diffusion method, respectively. In addition, TLC was done to profile the metabolites within the extracts using Dragendorffs reagent to identify the presence of alkaloids metabolite. The cytotoxicity result showed that the treatment of the extracts (100 mg/mL) inhibited the proliferation of HepG2 cells and the IC50 for all extracts exceeded 30 mg/mL indicating that the extracts were not cytotoxic to the cells. For antioxidant activity, all extract showed good antioxidant activity with the IC50 value obtained more than 50%. While, screening of bacterial properties using Gram-positive (Staphylococcus aureus, Bacillus cereus, Micrococcus sp.), and negative bacteria strains (Escherichia coli, Salmonella typhimurium, Pseudomonas aeruginosa) showed that all samples have antibacterial activity against Micrococcus sp only. The TLC profiling of A.planci and E. luzonicus showed the presence of alkaloids. Since the result found that A. planci, E. luzonicus, and E. calamaris have no cytotoxic activity against HepG2 cells (no IC50 value), further study such as anti-atherosclerosis potential agent can be evaluated. Subsequently, a very good antioxidant activity of all samples is good also to screen their potency as an anticancer agent against some cancer cells.
Key words: Acanthaster planci, Echinaster luzonicus, Echinotrix calamaris, cytotoxicity, antibacterial, antioxidant
|
