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Single Strand Conformation Polymorphism in Exon C of Estrogen Hormone Receptor Gene (ERα) in Murrah / Graded Murrah

Kathiravan Ramaiyan Selvaraju, Chitra Ramasamy, Murali Nagarajan, Arthanarieswaran Marappan.

Abstract
Buffaloes’ productivity is affected mainly due to many inherent disorders. Estrogen hormone is found to be a important hormone directly linked with reproductive functions of buffalo. The present study was performed with 203 Murrah / Graded Murrah buffaloes maintained at different locations. The Polymerase Chain Reaction- Single Strand Conformation Polymorphism (PCR-SSCP) of ER-α gene revealed monomorphic pattern for all the tested animals, which indicates the fixation of allele in tested buffalo population. As a conclusion, monomorphic pattern of Estrogen Receptor-α (ERα) gene is considered a unique feature that may be related to the characteristic of buffaloes.

Key words: Buffalo, PCR-SSCP, ERα gene, Monomorphism



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