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Original Article

pnr. 2013; 4(1): 66-70


Insignificant effect of cleistanthin A and cleistanthin B on motor function in animal models

Chavan Madhavrao, Subramani Parasuraman, Ramasamy Raveendran.

Abstract
Aim: To study the in vitro and in vivo effects of cleistanthin A and cleistanthin B on the cholinergic receptors and the motor function using mouse as an animal model. Materials and Methods: The cleistanthin A and cleistanthin B were isolated from the leaves of Cleistanthus collinus using column chromatography and purified. The effect of cleistanthins A and B on locomotor activity, motor co-ordination and grip strength in mice were evaluated using actophotometer, rota-rod apparatus and grip strength test respectively (in vivo). The effect of cleistanthins A and B on nicotinic acetylcholine receptor was studied using a mouse esophagus preparation (in vitro) and the concentration-response curves of carbachol and cleistanthins A and B were recorded using a data acquisition system (Biopac Inc. USA) through a variable transducer (500 g). Results: Cleistanthins A and B did not have any significant effect on locomotor activity, motor co-ordination and grip strength when they were used upto 400 mg/kg BW. Cleistanthins A and B did not show any significant inhibitory effect on nicotinic acetylcholine receptors in isolated mouse esophagus tissue preparation at 1, 3, and 10 μg doses. Conclusion: Cleistanthins A and B do not have significant activity on the motor system when used upto 400 mg/kg in mice and also they did not have any effect on nicotinic acetylcholine receptors when used upto 10 μg in the isolated mouse esophagus preparation.

Key words: Carbachol, cleistanthinA, cleistanthin B, nicotinic cholinergic receptor






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