In-vitro work was designed to determine antimicrobial and anti-sporulation effect of Zingiberspectabile, Piperbetle, Cissusquadrangularis, CostuspictusandCentellaasiaticaextracts on cloacal isolates and Eimeriaoocysts from vanaraja birds. Antibiogram of methanolic extracts of herbal extracts against salmonella and E.coli isolates were performed. Oocysts collected from naturally coccidiosis infected birds were treated with herbal extracts. The percentage of sporulation inhibition (SI) of oocysts was estimated. Extracts of Zingeberspectabile (510 µg) and Piper betle (557 µg) exhibited significantly highestantibacterial activitywith an average ZI of 15.92 mm againstE. coliisolates. Significantly highest ZI (16.6 mm) was reported with Zingeberspectabile(587 µg) and Piper betle(534 µg) against the Salmonella isolates. Cissus quadrangularis and Costus pictus recorded significantly lowest ZI of 6 mm against bothE.coliand Salmonella isolates. The extracts of Zingiberspectabile(root) and Piperbetle (leaf) inhibited sporulation of oocysts significantly higher (>80%) as compared to control and other treatments. The Piper betle (leaf) inhibited more than 80% of sporulation of caecaloocysts and 70% of sporulation was inhibited by Zingiberspectabile(root). Extracts of Zingiberspectabile(leaf) and Centellaasiatica showed statistically similar sporulation inhibitory action (>50%) on both intestinal and ceacaloocysts. Extracts of Zingiberspectabile(stem) showed more than 70% inhibitory action on intestinal oocysts and its inhibitory level on ceacaloocysts is less.The extracts of Cissusquadrangularis showed significantly less sporulation inhibitory action against both intestinal and caecalcoccidialoocysts. This study revealed that methanolic extracts of Zingeberspectabile and Piper betle have anti-bacterial properties against E.coli and Salmonellaof poultry and could be further explored for their anti microbial properties. Methanolic extracts of Zingeberspectabile, Piper betle, Centellaasiatica and Cissusquadrangularishave shown pronounced anti-sporulation activity on oocysts and thus they could be further studied and explored forfeed and water supplements asanticoccidial in poultry.
Herbal extracts, antibiogram, antisporulation, coccidial oocysts, rural poultry, A&N Islands
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