Review Article |
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The development of multiplication conditions in vitro of Stevia rebaudiana Bertoni in MoroccoBouaaza Ghizlane, Chetto Ouiam, Beniken Lhou, Dziri Laila, Benkirane Rachid, Hamid Benyahia. Abstract | | | | Stevia is an industrially and medicinally important plant used as a sweetener belonging to the family Asteraceae. The leaves of this plant contain a group of chemical compounds called steviol glycosides, which have been used as a natural sweetener substitute for sugar for a long time. The study aimed to develop a reproducible protocol for in vitro propagation of Stevia rebaudiana Bertoni. Nodal explants were cultivated on Murashige and Skoog (MS) medium supplemented with different concentrations of 6-Benzylaminopurine (BAP), Kinetin (Kin), and Indole-3-acetic acid individually or in combination for shoot induction. After a month, the leafy stems were placed in an MS medium supplemented with IAA and 1-Naphtalenacetic acid at 0.5 mg/L for rooting. MS medium without hormones (the control) produced the highest percentage of bud burst. The best treatment for shoots regeneration was MS medium supplemented with 1.5 mg/L BAP and 0.25 mg/L KIN. Medium with the highest multiplication rate was found to include MS fortified with 0.5 mg/L KIN. The medium MS supplemented with 0.5 mg/L IAA showed the maximum number of roots per shoot. About 93% of rooted plants were successfully acclimatized on pots containing peat (60%) and sand (40%).
Key words: micropropagation. Nodal explant. Self-incompatible. Stevia rebaudiana. seeds propagation, rooted plants.
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