Home|Journals|Articles by Year Follow on Twitter| Subscribe to List

Directory for Medical Articles
 

Open Access

Original Article

AJVS. 2017; 55(2): 1-9


Polymorphisms of TLR4 Gene and Its Association With Genetic Resistance to Salmonella Enteritidis Infection in Fayoumi Breed and Hy-line Strain in Egypt

Shymaa A. Khatab, Shabaan A. Hemeda, Abeer F. El-Nahas, Walaa S.H. Abd El Naby.

Abstract
The aim of this study is to screen for genetic resistance to different doses of salmonella infection and its possible association with polymorphisms in exon 1 and 2 of TLR4 in Fayoumi breed and Hy-line strain of chickens. Two experiments was done, first one for measuring the variation of TLR4 -exon 1 and exon 2 of different genotypes of chicken under study and resistance to salmonella enteritidis. This study include 100 birds of each breeds, were infected orally with LD50 (108 cfu) salmonella enteritidis at age of two weeks and was divided into two groups (susceptible and resistance). TLR4 gene was genotyped with PCR-RFLP, The genotyping results on exon 1 (596 bp) by Taq1 restriction enzyme showed that the BB genotypes is more resistance to infection in the Fayoumi breed and also the AB genotypes is more resistance to infection in the Hy-line strain. Also The genotyping result on exon 2 (793 bp) by EcoR1 restriction enzyme showed only one genotype BB in Fayoumi breed and The frequency of AB genotypes is more resistant to infection than BB genotypes in Hy-line strain. The second experiment to evaluate the variation in immune response to the different genotypes of TLR4 -exon 1 and exon 2 this study include 100 birds of each breeds were infected with (5 107 cfu) salmonella enteritidis and according to Elisa results the chickens devided into two groups (high and low immune response). The genotyping result on exon 1 (596 bp) by Taq1 restriction enzyme showed that the AB genotypes produce high immune response in both Fayoumi breed and Hy-line strain so can be used as a genetic marker for increase the immune response in chicks under study. Also The genotyping result on exon 2 (793 bp) by EcoR1 restriction enzyme showed only one genotype BB genotypes in Fayoumi breed and Hy-line strain.

Key words: Tol-like receptor, polymorphism, genetic resistance, salmonella enteritidis chicken


Full-text options

Full-text Article




Advertisement
Journal of Contemporary Medical Education

SUBMIT YOUR ARTICLE NOW




ScopeMed.com
BiblioMed Home
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.