This study was designed to evaluate the protective effects of Nigella sativa and (NS) and vitamin A (VA) on some serum biochemical parameters, and histological changes of the liver in CCl4 treated rats. Fifty male albino rats were randomly divided into five equal groups (10 rats each). The first group (G1) was used as a shame-exposed negative control. The other groups were orally treated with 1 ml/kg CCl4 (mixed with an equal volume of sesame oil) twice a week for 28 days except. The second group (G2) was used as a positive control. The third group (G3) was orally treated with 500 mg/kg BW/day of NS. The fourth group (G4) received orally treated with VA 75 mg/kg BW/day, where the fifth group (G5) received VA and NS with the same amount in G3 and G4 for 28 days. Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), Serum total bilirubin, urea, creatinine, total protein, albumin, Globulin, A/G ratio, TC (total cholesterol), TG (triglycerides), LDL-C and HDL-C levels were measured. Total protein, globulin and A/G ratio were significantly decreased in CCl4 treated rats while AST, ALT, ALP, total bilirubin, triglycerides total cholesterol, LDL-C and HDL-C levels were significantly increased (P < 0.01). Whereas non-significant change was observed in serum urea, creatinine and albumin levels as compared to control group (G1). Also, after treatment the CCl4-treated rats with NS (G3) and VA (G4), amelioration was observed in the previous parameters but not significant except in globulin and A/G ratio after treatment with VA. In addition, after treated CCl4 receiving rats with both NS and VA (G5), significantly changes were observed in the levels of total protein, globulin, A/G ratio, triglycerides and total cholesterol when compared with control group. The histopathological changes observed in the liver of the experimental groups were correlated with the changes in the biochemical parameters.
Key words: Carbon tetrachloride, Nigella sativa, vitamin A, antihepatotoxic activity
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